RNA sequencing analysis after MK5 knockdown in LATS1/2-null RPE1 cells

Transcriptional regulator YAP is activated in multiple human cancers and plays critical roles in tumor initiation, progression, metastasis, and drug resistance. However, therapeutic targeting of the Hippo-YAP pathway has been challenging due to its low druggability and limited knowledge of YAP regulation in cancer. Kinome siRNA library screening using an oncogenic YAP activation model identified the serine/threonine kinase MK5 as a positive regulator of YAP. To explore transcriptome signatures of MK5 activity, we performed RNA sequencing analysis after MK5 knockdown in LATS1/2-null RPE1 cells. Overall design: We performed RNA sequencing analysis from three biologic replicates of LATS1/2-null RPE1 cells transfected with either control or MK5 siRNAs for 48 hr.