Epitope mapping of MAbs to a panel of site mutations and truncations of SFTSV N protein tested by IFA assay.

a–dSingle alanine substitution at amino acid positions a5∼15 of N-terminal; bpositions 35∼45; cpositions 132∼142, dpositions 235∼245 of C-terminal. eRefer to major MAbs representing abolished or significantly reduced binding activity to mutation of E10A in N protein; (+) to (+++) indicates the relative intensity of fluorescence. R. Poly: a rabbit polyclonal antibody immunized with the SFTSV recombinant N protein.