Silencing branched-chain ketoacid dehydrogenase kinase exacerbates doxorubicin induced cytotoxicity in triple-negative breast cancer cells. Silencing branched-chain ketoacid dehydrogenase kinase exacerbates doxorubicin induced cytotoxicity in triple-negative breast cancer cells

TO BE FILLED IN WITH ABSTRACT Overall design: MDA-MB-231 cells were transfected with either of two siRNA’s targeting BCKDK (n=3 for each siRNA) or a non-targeting control (n=4) and cultured for 48 h before cells were harvested and RNA extracted using the Qiagen RNeasy mini kit according to the manufacturer’s instructions.