Effects of Genistein on LNCaP Gene Expression

Gene expression profiling to identify genes significantly modulated by low and high doses of genistein in LNCaP cells. Significant genes were identified using StepMiner analysis and significantly altered pathways with Ingenuity Pathways analysis. Genistein significantly altered expression of transcripts involved in cell growth, carcinogen defenses and steroid signaling pathways. The effects of genistein on these pathways were confirmed by directly assessing dose-related effects on LNCaP cell growth, NQO-1 enzymatic activity and PSA protein expression. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. SUBMITTER_CITATION: Suvarna Bhamre, Debashis Sahoo, Robert Tibshirani, David L. Dill and James D. Brooks. Gene expression changes induced by genistein in the prostate cancer cell line LNCaP. The Open Prostate Cancer Journal. 3: 86-98, 2010