Feline microRNA transcriptome in whole blood in 6 healthy cats and in 6 cats with preclinical hypertrophic cardiomyopathy

Aim to characterize differentially expressed miRNAS between healthy Norwegian Forest cats and healthy Domestic Shorthair cats, and to compare with cats with hypertropic cardiomyopathy (HCM)
Six neutered healthy cats, three male domestic cats (DOM) and one male and two female Norwegian Forest (NF) cats were included. Each healthy cat was matched (breed, sex, age, body-weight and body condition score) with a cat with HCM.
The dataset includes the miRDeep2 report, rawcounts miRNAs, differentially expressed miRNAs for contrasts compared using DESeq2, human and feline target genes producing messenger RNA (mRNA) and gene ontology analysis (GO) for these 12 cats.

Whole blood was collected in PAXgene blood RNA System frozen and stored in -20 °C until date of RNA-extraction for a median storage time of 177 days. Total RNA was extracted. Samples with an RNA integrity number (RIN)-value of 7.7 or higher were included in the study. Libraries were prepared and quantified and normalized prior sequencing. Paired-end sequencing data was generated. Bioformatic data processing and count genertion of known and novel miRNAs in cats were identified using miRDeep2. Mature miRNA and hairpin sequences were downloaded from miRBase with human as main reference, an mouse and dog assigned as close relatives. Main reference miRNAs identified in the dataset were classified as predicted known miRNAs, and miRNAs previously not described in the main reference were classified as novel miRNAs by miRDeep2. Only novel miRNAs with a miRDeep2 score of >5.0 was included in the count-file generated for subsequent differently expressed (DE)-analyses. Identification of DE miRNAs were performed in DESeq2.
Number of variables 6: breed, age, sex, body-weight, body condition score, healthy or hypertrophic cardiomyopathy.
The compiled files gives an overview of the data set.
Compiled Excel file “miRDeep2_counts_DEsignmiRNAcontrasts_compiled” is a compiled file with the miRDeep2 report, raw counts of miRNAs and the differentially expressed miRNAs found in the dataset. The following nine csv-files are named miR_ and the name of the file in the compiled Excel file.
Compiled Excel file “Target_HumanGOmiRNA_Feline_GOmiRNA_compiled” is a compiled file with the human and feline target genes producing messenger RNA for the differentially expressed miRNAs found in the dataset, gene ontology analysis of these miRNAS both for human and feline genes. The following nineteen csv-files are named Target_ and the name of the file in the compiled Excel file.

本数据集旨在表征健康挪威森林猫（Norwegian Forest cats）与健康短毛家猫（Domestic Shorthair cats）之间的差异表达miRNA，并与肥厚型心肌病（hypertrophic cardiomyopathy, HCM）患猫进行对比分析。

本研究共纳入6只已绝育健康猫，其中3只雄性短毛家猫（DOM），以及1只雄性、2只雌性挪威森林猫（NF）。每只健康猫均在品种、性别、年龄、体重及体况评分维度上与一只HCM患猫进行匹配。

本数据集包含miRDeep2 (miRDeep2)分析报告、miRNA原始计数数据、使用DESeq2 (DESeq2)进行组间对比得到的差异表达miRNA数据，以及针对这12只猫鉴定得到的编码信使RNA（messenger RNA, mRNA）的人类与猫科靶基因，同时包含上述靶基因的基因本体论（gene ontology, GO）分析结果。

采用PAXgene血液RNA系统（PAXgene blood RNA System）采集全血样本，将其冷冻保存于-20℃直至RNA提取，样本中位存储时长为177天。随后提取总RNA，仅纳入RNA完整性数（RNA integrity number, RIN）≥7.7的样本进入后续实验流程。构建测序文库并完成定量与归一化后进行测序，最终生成双端测序数据。使用miRDeep2完成生物信息学数据处理，并鉴定猫科已知与新型miRNA的计数结果。从miRBase下载成熟miRNA与发夹结构序列，以人类作为主要参考物种，小鼠与犬作为近缘参考物种。本数据集中鉴定得到的主要参考miRNA被归类为预测已知miRNA，而miRBase中未记载的miRNA则被miRDeep2归类为新型miRNA。仅miRDeep2评分>5.0的新型miRNA被纳入后续差异表达（differentially expressed, DE）分析的计数文件中。差异表达miRNA的鉴定通过DESeq2完成。

本数据集共包含6类变量：品种、年龄、性别、体重、体况评分以及健康/肥厚型心肌病状态。

汇总文件可对本数据集进行整体概览。名为"miRDeep2_counts_DEsignmiRNAcontrasts_compiled"的汇总Excel文件包含miRDeep2分析报告、miRNA原始计数数据以及本数据集鉴定得到的差异表达miRNA。该汇总文件拆分为9个以"miR_"开头、文件名与汇总Excel文件内文件名一致的CSV文件。

名为"Target_HumanGOmiRNA_Feline_GOmiRNA_compiled"的汇总Excel文件包含本数据集鉴定得到的差异表达miRNA对应的编码信使RNA的人类与猫科靶基因，以及针对这些miRNA的人类与猫科靶基因的基因本体论分析结果。该汇总文件拆分为19个以"Target_"开头、文件名与汇总Excel文件内文件名一致的CSV文件。