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Supplementary Tables: Effect of mismatch between types of viral nucleic acid and intended targets of extraction kits on PCR-based testing

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Figshare2022-06-28 更新2026-04-28 收录
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Supplementary Table 1. The summary of the designed primer sets information for the detection of respective viruses Supplementary Table 2. Composition of the reaction mixture (in µL) for conventional PCR assays performed on nucleic acid extracted from bovine herpesvirus type 1 (BHV-1), porcine epidemic diarrhea virus (PEDV), porcine circovirus type 2 (PCV-2), and porcine rotavirus type A (PoRVA) Supplementary Table 3. PCR conditions for conventional PCR assays performed on nucleic acid extracted from bovine herpesvirus type 1 (BHV-1), porcine epidemic diarrhea virus (PEDV), porcine circovirus type 2 (PCV-2), and porcine rotavirus type A (PoRVA) Supplementary Table 4. Composition of the reaction mixture (in µL) for real-time (r) PCR assays performed on nucleic acid extracted from bovine herpesvirus type 1 (BHV-1), porcine epidemic diarrhea virus (PEDV), porcine circovirus type 2 (PCV-2), and porcine rotavirus type A (PoRVA) Supplementary Table 5. PCR conditions for real-time (r) PCR assays performed on nucleic acid extracted from bovine herpesvirus type 1 (BHV-1), porcine epidemic diarrhea virus (PEDV), porcine circovirus type 2 (PCV-2), and porcine rotavirus type A (PoRVA) Supplementary Table 6. Summary of the amplification sensitivity results of real-time (r) PCR assays
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2022-06-28
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