Supporting Geochemical Data from Studies Investigating Pharmaceutical Biotransformation by a Biomat that Colonizes Open-Water Unit Process Wetlands

These workbooks provide supporting field and experimental geochemical data from studies investigating pharmaceutical biotransformation by a benthic biomat community that colonizes shallow, open-water unit process wetlands. Field data consists of diel and depth-resolved sampling of a coupled water column and benthic biomat system within a mature shallow, open-water wetland: (1) biomat oxygen profiling from October 2018, (2) biomat porewater profiles from September and October 2018, (3) gas flux measurements at the air-water interface overlying biomat from October 2018. Experimental data comes from microcosms inoculated with Prado Wetlands biomat and wetland water, then subjected to oxic or anoxic conditions with and without enzyme inhibitors (targeting nitrogen cycling processes or methane monooxygenase); oxic microcosms were also subjected to light or darkness in the absence of methane or inhibitors to query the effect of photosynthetic conditions. Microcosms were amended with a suite of environmentally relevant pharmaceuticals and chemical constituents were tracked through time. Pharmaceutical compounds were analyzed at the University of California, Berkeley, whereas nitrogen species, methane, carbon dioxide, sulfate, and pH were analyzed at the Colorado School of Mines.