RNA-Sequencing Analysis of ox-LDL-treated Peritoneal Macrophages Transcriptomes

Since previous stduies demostrated that oxidized phospholipids function as caspase-11 agonists to induce noncanonical inflammasome activation in immune cells and the levels of oxidized phospholipids derived from ox-LDL are largely elevated in atherosclerotic lesions. Purpose:RNA-Sequencing analysis of ox-LDL-treated peritoneal macrophages transcriptomes.Methods and results:The principal component analysis gene expression profiles of the control and ox-LDL-treated groups were clearly distinct. Among the DEGs that met the cutoff criteria of a -log10(false discovery rate (FDR)) > 2 and |log2(fold change (FC)| > 2. A total of 1,388 downregulated and 855 upregulated genes were identified. GSEA showed that the dominant upregulated pathways in ox-LDL-treated macrophages were associated with the IL-1-mediated signaling pathway, response to cytokine stimulus, and granulocyte migrationwe discovered that caspase-11-mediated inflammation signaling was significantly activated in ox-LDL-treated peritoneal macrophages.Conclusions:we verified caspase11associated inflammatory signaling was significantly activated in ox-LDL-treated macrophages. Overall design: WT mice peritoneal macrophages were isolated on the third day after thioglycollate infusion and then primed with LPS (100ng/ml) followed by 16 hours of ox-LDL(100ug/ml) stimulation .Total RNA was extracted from 5 biological replicates for each group.