In vivo transcriptomic responses to thioacetamide exposure in guinea pig liver tissue

In this study we tested the ability to predict organ injury from transcriptomic data in Hartley guinea pigs at early time points after exposure to thioacetamide (9 and 33 hours). We selected thioacetamide, a compound extensively used in animal studies for its ability to cause liver damage. We treated 30 Hartley guinea pigs with saline solution (control), 25 mg/kg (low dose), and 100 mg/kg (high dose) to produce different degrees of injury. RNA samples for gene expression analysis were collected from the liver at 9 and 33 hours. Number of replicates were five.