Effects of RofA and the modified intergenic region, on FCT-region gene transcription, as determined by qRT-PCR.

*Each mid log and stationary phase value is based on at least 4 different RNA preparations; not all genes were tested with every RNA preparation; some measures were repeated in additional experiment(s) with new cDNA. The data for late log phase are based 3 different RNA preparations, with 1 or 2 cDNA preparations, tested for a total of 6 to 8 times. Ratios >2-fold are highlighted in bold.**Average for each RNA preparation from Alab49 rofA::aad9 cultures, used to generate the cumulative qRT-PCR data.?The t-value is based on the transcript abundance ratio relative to wt Alab49, for mid log versus stationary phase cultures (unpaired, 2-tailed t-test). NS, not significant; n.d., not determined.