Polysome profiling analysis of myeloid progenitors of control mice or mice conditionally deficient for Elp3 in the hematopoietic system

Aim of the experiment was to compare the genes enriched in the heavy polysome fraction of myeloid progenitors originating from control Elp3fl/fl mice, and in myeloid progenitors originating from mice that underwent hematopoietic cell-restricted deletion of the catalytic subunit Elp3 of the Elongator complex (Elp3HscKO mice). The Elp3fl/fl strain was generated in house and first described in (DOI: 10.1084/jem.20142288). Littermates of 8-12 weeks old were used in all experiments.本实验旨在对比两组髓系祖细胞（myeloid progenitors）中富集于重度多聚核糖体组分（heavy polysome fraction）的基因：一组来源于对照Elp3fl/fl小鼠，另一组来源于造血细胞特异性敲除延伸因子复合物（Elongator complex）催化亚基Elp3的Elp3HscKO小鼠。Elp3fl/fl品系由本实验室自主构建，首次报道于文献DOI: 10.1084/jem.20142288。所有实验均采用8-12周龄的同窝小鼠。