Transcriptomic analysis of the apicomplexan parasite Goussia janae. Goussia janae Transcriptome

To shed light on the biology of the apicomplexan parasite, Goussia janae, isolated from chub (Squalius cephalus), total RNA extracted from exogenously sporulated oocysts was sequenced on a Illumina HiSeq 2500. De novo transcriptome assembly was done by the Trinity pipeline and annotation of the assembled transcripts by the Trinotate pipeline. Phylogenetic and interpretive analyses from the RNASeq data provided novel insight into the metabolic capabilities, the composition of the invasive machinery and the phylogenetic relationships of this parasite of cold-blooded vertebrates with other coccidians. This allows re-evaluation of the phylogenetic position of G. janae and sheds light on the emergence of the highly successful obligatory intracellularity of apicomplexan parasites.