IgG-Seq of severe myalgic encephalomyelitis patients and matched healthy household controls

IgG-Seq was used to identify stool microbes which are reactive to serum immunoglobulin G (IgG) in severe myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) patients (n=5) and matched household controls (n=5). Metagenomic sequencing was undertaken on stool microbes separated into “IgG positive”, “IgG negative” and “all” fractions on a fluorescent activated cell sorter. IgG binding to taxon's was scored using the IgG probability ratio which was adapted from the IgAScores R package.