Effect of Shiga toxin (Stx) and glucosylceramide synthase inhibitor (Gb3i) treatments on SV40 transformed human kidney endothelial cells. Effect of Shiga toxin (Stx) and glucosylceramide synthase inhibitor (Gb3i) treatments on SV40 transformed human kidney endothelial cells

To investigate the transcriptional changes caused by Stx1 and Stx2 in human kidney microvascular cells (HKMECs), and the effects of GCSi pre-treatment on Stx-mediated effects Overall design: SV40-transformed HKMECs were grown to confluency in cell culture plates, with subsequent treatment with GCSi or vehicle, and then treated with either Stx1, Stx2 or vehicle.