Global hepatic gene expression data from PPARa liver KO, PPARa liver WT, PPARaKO and WT male mice treated or not with Fenofibrate

Fenofibrate is a specific agonist of the nuclear receptor PPARa. To identify the gene expression under the strict dependence of hepatic PPARa activity, we generated a new mouse strain of PPARa-specific deletion in hepatocyte (albumin-Cre+/- Pparaflox/flox or LKO) and we compared them to total Ppara KO (KO), wild-type (WT) and liver WT (albumin-Cre-/- Pparaflox/flox or LWT) mice. We used microarrays to detail the global programme of gene expression in liver of Ppara LKO, LWT, Ppara KO and WT male mice. There are 36 liver samples, each from an individual mouse. The samples are from Ppara liver KO (LKO), Ppara KO (KO), wild-type (WT) and liver WT (LWT) male mice of 14 week-old from the same genetic background (C57Bl/6J) treated with Fenofibrate (100 mg/kg/day) or vehicle (aqueous solution of gum Arabic 3%) by daily gavage for 10 days. n= 4 mice for LKO, LWT and WT genotypes treated with vehicle; n=3 for KO mice treated with vehicle; n=5 mice for LWT, LKO and KO genotypes treated with fenofibrate; n=4 WT mice treated with fenofibrate. All mice were sacrified at ZT14.非诺贝特（Fenofibrate）是核受体PPARα的特异性激动剂。为鉴定严格依赖肝脏PPARα活性的基因表达谱，我们构建了肝细胞特异性敲除PPARα的新型小鼠品系（albumin-Cre+/- Pparaflox/flox，简称LKO），并将其与全身Ppara敲除小鼠（KO）、野生型小鼠（WT）以及肝脏野生型小鼠（albumin-Cre-/- Pparaflox/flox，简称LWT）进行对照分析。我们使用微阵列（microarrays）解析了Ppara LKO、LWT、Ppara KO及WT雄性小鼠肝脏内的全局基因表达谱。本数据集共包含36份肝脏样本，每份均来自单只个体小鼠。所有样本均来源于同一遗传背景（C57Bl/6J）的14周龄雄性小鼠，实验分组包括Ppara肝脏特异性敲除（LKO）、全身Ppara敲除（KO）、野生型（WT）及肝脏野生型（LWT）四组，小鼠均经每日灌胃给予非诺贝特（100 mg/kg/天）或赋形剂（3%阿拉伯胶水溶液），持续给药10天。各组样本量如下：赋形剂处理的LKO、LWT及WT基因型小鼠每组n=4；赋形剂处理的KO小鼠n=3；非诺贝特处理的LWT、LKO及KO基因型小鼠每组n=5；非诺贝特处理的WT小鼠n=4。所有小鼠均于ZT14时处死。