FBXW7 is a defining driver of uterine carcinosarcoma by promoting epithelial-mesenchymal transition

Fbxw7 is a tumor supressor frequently mutated in endometrial cancer. To analyze the signaling pathways that can be altered by Fbxw7 we compared RNA expression in the presence or absence of Fbxw7 in mouse endometrial cancer cells deficient for Fbxw7 and Pten. Cells (UCS1 and UCS2) were isolated from mice deficient in Fbxw7 and Pten in endometrial epithelium and harboring uterine carcinosarcoma. UCS1 and UCS2 cells were transduced with A) lentivirus expressing Fbxw7 under a Tet inducible promoter (pLVX-FBXW7) or B) lentivirus with vector only (pLVX) AND c) with lentivirus expressing TetON. Cells were selected with puromycin (pLVX) and G418 (tetON). RNA was isolated from biological replicates, RNA levels quantified and compared.