LPS genotype identification and mutations in the four closely related Burkholderia species.

Note: LPS genotypes and mutations were identified in the main O-antigen biosynthesis gene locus and the oacA homologs. Frame-shifted mutations of the O-antigen biosynthesis genes were found in their wbiI(*), wbiF(†),wbiE (‡), and wbiD (ψ) genes. We did not test the effects of wbiF mutant in B. pseudomallei 14, and wbiE mutant in B. pseudomallei B7210, due to unavailability of live bacterial cultures. The listed GenBank accession numbers are associated with the LPS genotype identification, not for oacA analysis. Details of the oacA mutations are demonstrated in Figure S1.