Calculated EMAST mutation rates for <i>D8S321</i> and <i>D20S82</i> in various MMR backgrounds.

Data from the EGFP-positive population at weeks 4, 5, and 6 time points were used for -4 bp frameshift mutation rate analysis. Single mutation rates were calculated by combining and averaging time-specific mutation rates. Rates are expressed as mutations at microsatellite sequence per cell per generation. Data shown are mean±SEM. a, b, c, and d represent significant difference in the mutation rate (P<0.05). ahMLH1−/− vs. MMR-proficient (D8S321 P = 0.00012, D20S82 P = 0.01069); bhMLH1−/− vs. hMSH6−/− (D8S321 P = 0.00012, D20S82 P = 0.01021); chMSH3−/− vs. MMR-proficient (D8S321 P = 0.00008, D20S82 P = 0.01525); dhMSH3−/− vs. hMSH6−/− (D8S321 P = 0.0001, D20S82 P = 0.01415). Mutations rates were calculated based on the “method of the mean” developed by Luria and Delbruck [25].