Transcriptomic analysis of class I odorant receptor enhancer deletion [eB]

We used RNAseq based transcriptome analysis to compare gene expression changes in the olfactory mucosa resulting from CRISPR-based deletion of a putative enhancer in the class I odorant receptor gene cluster. 6 control and 6 enhancer deletion animals. Age matched. Deletion eB = 1,155 bp (chr7:102,847,692-102,848,846)我们采用基于RNA测序（RNA-seq）的转录组分析技术，对比分析I类气味受体基因簇内推定增强子经CRISPR介导敲除后，嗅黏膜中的基因表达变化。本实验共纳入6只对照动物与6只增强子敲除动物，所有受试动物均为年龄匹配个体。本次敲除的eB区域长度为1155 bp，位于7号染色体：102,847,692-102,848,846。