Quantification Figure 4 (GraphPad Prism file)

Figure 4 – Cytokines expression in cell extracts from human iPSC-derived astrocytes. (a) – Interleukin-6; (b) – Interleukin-1 beta and (c) – TNF-α expression was assessed 24 h after exposing cells to vehicle or 10 ng/mL TNF-α. Data are presented as means ± SEM of fold change for (a) and (b) and Ct (c) since the basal levels of TNF-α were below the limit of quantification in non-stimulated cells. Experiments were performed in triplicates from 4 cell lines. *P < 0.05; N.D. non detected. Unpaired Student´s t-test.