Label-free single-cell RNA Multiplexing leveraging genetic variability [scRNA-seq]

Single cell RNA sequencing has enabled unprecedented insights into the molecular cues and cellular heterogeneity underlying human disease. However, the high costs and complexity of single cell methods remain a major obstacle for generating large scale human cohorts. Here we compare current state-of-the-art single cell multiplexing technologies, and provide a new widely applicable demultiplexing method, SNP-Fishing, that enables simple, robust high-throughput multiplexing leveraging genetic variability of patients. Single-cell and single-nuclei RNA-sequencing were pooled from different patients and demultiplexed with matching bulk-RNA-seq of the same individuals.