Measured vascular volumes (Vv) in naïve and anti-VEGF-administered mice.

Note: Literature (Lit.) values are listed for comparison. Nude mice (n = 5) were naïve or administered a single, intravenous bolus dose (10 mg/kg) of the cross-species anti-VEGF antibody [12], B20-4.1, 24 h prior to assay. All values are reported in µL/g. Values of Vv were measured using both direct and indirect RBC labeling methods [2]. Note that the assay method can greatly influence measured Vv, especially in probe clearance organs.a[39].b[5].c[3].dMeasured using 3H-inulin.eFor well-perfused organs 10% vascular space is assumed [5].fEstimated from experimental IgG data at early time points [5].gEstimated from human data.hData reproduced with permissions [2].