Genome sequencing with short reads

To test methods for de novo genome assembly using Illumina and Pacific Biosciences shotgun data, microbial genomes with finished reference sequences were selected. Streptococcus pneumoniae TIGR4 was one of these microbial genomes. At least one Pacific Biosciences library (long fragment) and two Illumina libraries (short fragment and jumping) were generated and sequenced. Genomic DNA from Claudette Thompson. Two different genome assemblies are part of this project. The assembly in AKBW00000000 is the complete chromosome assembled from Illumina reads. The assembly in AKVY00000000 is the complete chromosome assembled from reads used to assemble AE005672 plus reads from a different isolate of the strain.