Summary of strains and oligos used in this study.

Strains used in this study are listed with unique identifiers (SGM-#). For E. coli donor strains, the primers or gene block used to construct each plasmid are listed in the “Oligos” column. Genetic changes introduced into V. cholerae were screened using the method indicated in the “confirmation” column: either via PCR using the indicated primers (SM-#), via purification on kanamycin plates, or via blue-white screening on X-gal plates. (XLSX)