RNA sequencing of Bifidobacterium pseudocatenulatum MP80 grown on Lactose 2'-FL and LNFP-1

RNA sequencing revolutionized the bacterial gene expression analysis. The objective of this study was to identify the genes involved in metabolism of 2'-FL and LNFP1 in Bifidobacterium pseudocatenulatum MP80. We have obtained a list of genes upregulated in Bifidobacterium pseudocatenulatum MP80 when it is grown in 2% 2'-FL and LNFP1. Lactose grown samples were used as the control. Illumina RNAseq was conducted on RNA extracted from four samples of Bifidobacterium pseudocatenulatum MP80 (n=4) grown in 2% Lactose, four samples of Bifidobacterium pseudocatenulatum MP80 grown in 2% 2'-FL and four samples of Bifidobacterium pseudocatenulatum MP80 grown in 2% LNFP1. Samples were takin at the mid log phase of the growth curve.