Single-cell analysis of mouse plasmacytoid dendritic cells (pDCs) unravels their activation trajectory and their molecular regulation in vivo during a viral infection [FB5P-seq_C57Bl6]

The aim of the project is to understand the heterogeneity of murine plasmacytoid dendritic cells (pDC) for interferon I production. For this end we want to perform RNAseq on splenic pDC at ground state and also after infection of mice with MCMV at different time points. This dataset contains data from pDC sorted from wt C57Bl/6 mice, infected or not with Murine CytoMegalo virus (MCMV). Single-cells were FACS-sorted into 7 96-well plates (plates 1 and 2 : non-infected, plates 3 to 7 : infected) and analyzed by 5'-end single-cell RNAseq following the FB5P-seq protocol.