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METHODS AND COMPOSITIONS FOR INCREASING EFFICIENCY OF TARGETED GENE MODIFICATION USING OLIGONUCLEOTIDE-MEDIATED GENE REPAIR

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国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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PROBLEM TO BE SOLVED: To provide improved methods for modification of genes in plant cells, and plants and seeds derived therefrom. SOLUTION: The invention relates to increased efficiency of targeted gene mutation by combining gene repair oligonucleotides with approaches that enhance availability of components of target cell gene repair mechanisms, by introducing a gene repair oligonucleobase (GRON)-mediated mutation into a target deoxyribonucleic acid (DNA) sequence in a plant cell in the presence of antibiotics of bleomycin family. SELECTED DRAWING: Figure 1
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国家林业和草原科学数据中心
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2023-02-12
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