'Nonpareil' almond genome and methylome sequencing

Breeding and improvement efforts worldwide have led to the development of key, productive cultivars, including Nonpareil, which is the most commonly grown almond cultivar. Thus far, genomic resources for this species have been limited, and the whole genome assembly for Nonpareil is not currently available despite its economic importance and use in almond breeding. We generated a 615.89X coverage sequence using Illumina, PacBio, and optical mapping technologies. Gene prediction revealed 27,487 genes using MinION Oxford nanopore and Illumina RNA sequencing, and genome annotation found that 68% of predicted models are associated with at least one biological function. In addition to the genome sequence and annotation, this report also provides the complete methylome of several key almond tissues, including leaf, flower, endocarp, mesocarp, fruit skin, and seed coat.