High-throughput sequencing of circRNAs in ovarian cancer SKOV3 cell line

Circular RNAs(circRNAs) are conservative and stable and have been extensively studied in cancer in recent years. Expression profile of circRNA is largely unknown in ovarian cancer. This study explored circRNAs in the ovarian cancer cell line SKOV3. We injected untreated SKOV3 ovarian cancer cells into nude mice peritoneal cavity to mimic the circRNAs formation and degradation during tumor cell growth. Tumor tissues with good growth were obtained from nude mice, and high-throughput sequencing was performed to obtain circRNAs stably expressed in all samples.