Effects of age and sex on gene expression in the mouse heart

To compare the effects of biological sex (female/male) and age (4 mo./20 mo.) on C57BL/6J mouse hearts, we performed mRNA sequencing. Comparative gene expression analysis in male, female mouse hearts from 4 mo. and 20 mo. C57BL/6J mouse hearts (n=3 per group) using short-read Illumina polyA+ mRNA sequencing on a NovaSeq 6000 platform为探究生物性别（biological sex，雌性/雄性）与年龄（4月龄/20月龄）对C57BL/6J小鼠心脏的影响，我们开展了mRNA测序（mRNA sequencing）实验。针对4月龄与20月龄C57BL/6J小鼠的雌雄心脏组织（每组n=3），采用短读长Illumina polyA+ mRNA测序技术，在NovaSeq 6000测序平台上完成了比较基因表达分析。