Spatial transcriptomics based characterization of spatial architecture in human multiple primary lung cancer

Multiple primary lung cancers (MPLC) pose diagnostic and therapeutic challenges in the clinic. Here, we orchestrated the cellular atlas and spatial architecture of MPLC based on spatial transcriptomics. Overall design: We collected three MPLC patients (one squamous carcinoma and twk adenocarcinomas); each patient had two separate lung tumor lesions. Spatial transcriptomics sequencing was applied on 12 samples, including paired tumor and tumor-adjacent normal tissues from each of the two different tumor lesions of three MPLC patients (P1, P2, P3).