Differential Effects of Exercise Intensity on Gut Microbial Outcomes in Aging SAMP6 and SAMR1 Mice

Thirty-six male SAMP6 and twelve SAMR1 mice (5- and 7-month-old) were assigned to eight groups (n=6/group). Sedentary controls included age-matched SAMP6 and SAMR1 mice. Seven-month-old SAMP6 mice underwent 8 weeks of treadmill training: low-intensity continuous (12 m/min), moderate-intensity continuous (15 m/min), high-intensity interval training (HIIT; 12/20 m/min), or progressively intensified protocol. Fecal samples were collected post-intervention for 16S rRNA sequencing (V3-V4 region).