Genome wide differential gene expression analysis of MYCN-amplified neuroblastoma cells after lncNB1 knockdown with siRNAs. Genome wide differential gene expression analysis of MYCN-amplified neuroblastoma cells after lncNB1 knockdown with siRNAs

We identified that knocking lncNB1 reduces N-Myc protein stability, therefore, we performed affymetrix micoarray studies to investigate whether it was a direct or indirect effect. lncNB1 stabilizes N-Myc protein expression through activating DEPDC1B gene transcription. Overall design: We performed Affymetrix gene_array and Clariom_S_array in BE(2)-C cells after transfection with Control siRNA, lncNB1 siRNA-1 or lncNB1 siRNA-2 for 40 hours.