Total RNA sequencing of prospective axial stem cell cultures derived from human pluripotant stem cells. Total RNA sequencing of prospective axial stem cell cultures derived from human pluripotant stem cells

Here we describe a protocol for converting pluripotent stem cells to stable bi/multipotent stem cell populations with axial progenitor characteristics. To analyse the global transcriptome of the derived lines, we performed RNA-sequencing of 3 different axial stem cell lines Overall design: total RNA sequencing of three stable cell lines at passage 9 and undifferentiated parental hESC line H9