Multiple RNA structures affect translation initiation and UGA redefinition efficiency during synthesis of selenoprotein p

Mice carrying genomic deletions of SECIS1 or SECIS2 in the Selenop gene were euthanized and liver tissue was harvested and processed for RNA-Seq and ribosome profiling. From these studies, we determined the affect of SECIS1 and SECIS2 on UGA redifinition across the Selenop gene. Gene expression analysis of liver tissue from mice carrying genomic deletions of the 3' UTR SECIS1 or SECIS2 elements of Selenop针对在硒蛋白P（Selenop）基因中携带硒半胱氨酸插入序列（SECIS）1或SECIS2基因组缺失的小鼠，研究人员对其实施安乐死后采集肝组织，并开展RNA测序（RNA-Seq）与核糖体谱分析（ribosome profiling）。通过上述实验，我们明确了SECIS1与SECIS2对Selenop基因中UGA密码子重编码的影响。本研究同时对携带Selenop基因3'非翻译区（3' UTR）SECIS1或SECIS2元件基因组缺失的小鼠的肝组织进行了基因表达分析。