Mosquito pathogen status (DP1.10041.001)

A set of up to 1000 individual female mosquitoes per species per site per year are targeted for pathogen testing of arboviruses within the families Bunyaviridae, Alphaviridae, and Flavivirdae. From 2013-2019, top-priority mosquito species sent for pathogen testing included: *Aedes aegypti*, *Aedes albopictus* (tested if >100 collected per site-year), and *Culex tarsalis*, *Culex pipiens*, and *Aedes triseriatus* (tested if >200 collected per site-year). From 2013-2019, other individuals, particularly those identified to the species-level within the genera of *Aedes* and *Culex*, were tested if >200 were collected per site-year. Beginning in 2020, the list of vector species sent for pathogen testing was revised and shortened due to low infection prevalence. A smaller set of vectors that reach the minimum abundance threshold of 200 per site-year are selected for pathogen testing from the following species list: *Culex tarsalis*, *Culex restuans*, *Culex pipiens*, *Culex quinquefasciatus*, *Culex erraticus*, *Culex nigripalpus*, *Culex salinarius*, *Aedes japonicus*, *Aedes triseriatus*, *Culiseta melanura*, *Culiseta morsitans*, *Psorophora columbiae*, *Coquillitidia perturbans*, *Aedes dorsalis*, *Aedes trivittatus*, *Aedes canadensis mathesoni*. Instrumentation: