The comparison of cell biology features of glioblastoma cell cultures.

The characteristics of cells in different culture conditions are depicted separately: neoplastic cells from cultures which provided a stable cell line (blue bars), neoplastic cells from cultures that did not provide a cell line (red bars) and normal cells from the latter cultures (green bars). (A) The differences in the tumor cell content in glioblastoma cell cultures; (B) The comparison of the percentage of the cells incorporating BrdU during a 7-day incubation; (C) The comparison of the percentage of senescent (positive for SA-β-Gal) cells; (D) The results of Mann-Whitney U test. Ser E – p0-p3 (1–3 weeks) under classical conditions; Ser L – p6-p13 (8–16 weeks) under classical conditions; SF E – p0-p1 (1–3 weeks) under serum-free monolayer conditions; SF L – p3-p5 (6–16 weeks) under serum-free monolayer conditions; Sph E – t0-t2 (up to 3 weeks) of spheroid culturing; Sph L – t4-t8 (3–5 months) of spheroid culturing; E – early passages/transfers; L – late passages/transfers; T – tumor cells, expressing TP53 or EGFR and GFAP; N – normal cells; s – cultures providing stable cell lines, u – cultures not providing stable cell lines.