Expression of 97 surface markers and 462 mRNAs in 31586 cells from 15 leukemic human bone marrow

Seurat v3 object

ASSAYS:

AB: Antibody expression data

RNA: mRNA expression data

integrated: Antibody and mRNA expression matrices were concatenated and data was integrated across patients using scanorama

DIMENSIONALITY REDUCTION

MOFA: Multi-OMICS factor analysis to integrate AB and RNA data. MOFA was run on scanorama-integrated data and served as input for clustering and further dimensionality reduction.

MOFAUMAP: UMAP performed on MOFA dimensions.

Projected: Cell projected into the MOFAUMAP space of a healthy dataset. Display used in the manuscript.

METADATA

ct: Cell type

Batch: Source individual (AML1-3, AML Q1-Q6, APL Q1-Q6)

Patient Identifier age gender Genotype

AML Q1 AML116 71 f FLT3-ITD,NPM1-mut

AML Q2 AML327 62 f FLT3-wt,NPM1-mut

AML Q3 AML127 56 f FLT3-wt,NPM1-mut

AML Q4 AML314 51 m FLT3-ITD,NPM1-mut

AML Q5 AML334 78 m FLT3-wt,NPM1-mut

AML Q6 AML183 61 m FLT3-wt,NPM1-mut

AML1 AML1 44 f FLT3-ITD,NPM1-mut

AML2 Q59 61 f FLT3-wt,NPM1-mut

AML3 AML3 55 m FLT3-ITD,NPM1-mut

APL Q1 APL224 70 f APL

APL Q2 APL223 54 f APL

APL Q3 APL142 66 m APL

APL Q4 APL147 45 m APL

APL Q5 APL124 32 m APL

APL Q6 APL218 46 f APL

Changelog

v2: Objects were slimmed to correspond to the information described in our study. Data now only contains relevant dimensions reductions and metadata columns; unused RNA and antibody targets were excluded from the objects.