RNA-sequencing of Candida albicans RAP1-deletion mutant in low iron

The goal is to explore the functions of the transcription factor Rap1 in Candida albicans in low iron environment. The wild-type strain (SC5314) and the RAP1-deletion mutant strain were cultured in YPD medium with 400 micromolar of iron chelator bathophenanthrolinedisulfonic acid (BPS) for 24 h, and subsequently cultured in YPD medium with 200 micromolar of BPS for 5 h. Cell were then collected to assess the differences in the gene expression.