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Dataset supporting Figure 4 in "Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice"

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NIAID Data Ecosystem2026-05-01 收录
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https://figshare.com/articles/dataset/Dataset_supporting_Figure_4_in_Pan-cortical_2-photon_mesoscopic_imaging_and_neurobehavioral_alignment_in_awake_behaving_mice_/25114509
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Contains data from a single 1-photon widefield imaging experiment and a single Thorlabs mesoscope 2-photon imaging session from a dorsal mount mouse, corresponding to Figure 4. Included files contain imaging data, behavioral data, and python files with combined neurobehavioral data. Note that session names have the following format: "mouse#_bigEndianDate_cage#_info_session#_attempt#". Raw mesoscope imaging data is included in ScanImage rendered format as single big tiffs with the following nomenclature: "filename_2D.tiff". Mouse face and body cam images are included as standalone or concatenated .avi movie files, and behavioral data is included both as Spike2 files (smrx) and in exported form at Matlab data files (.mat). In all cases the first frame of the 2-photon movie, the right face/body movie, and Spike2 data are aligned to the first Labview-issued frameclock trigger (also recorded in Spike2, along with all other frameclock events). 2-photon triggers were sometimes incorrectly recorded in Spike2 (generally we recorded these as both events and waveforms), but were in all cases additionally exported from ScanImage tiff metadeta as timestamps (csv files ending in header.csv). Session start-time timestamps, also exported from ScanImage tiff metadata, appear as .txt files ending in "_starttime.txt". Preprocessed data (python) can be found in npy files with various names, each containing different subsets of variables relevant to the analysis. For each session, the npy file containing the string "standard_frames" contains the most complete, final stage set of preprocessed neurobehavioral data (in combined DataFrame format, exportable to nwb), including CCF/MMM alignments. The file containing the string "nb_dump" contains a large set of auxilliary variables that may be needed for additional preprocessing. Additional image files (tiff, png) and excel worksheets (xlsx, csv) containing high-level data summaries and records of intermediate analysis steps are also included. Please contact the authors for any additional clarifications as needed. See related materials in Collection at: https://doi.org/10.25452/figshare.plus.c.7052513
创建时间:
2024-02-01
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