GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation

Osteoporosis is a common systemic skeletal disorder resulting in bone fragility and increased fracture risk. It is still necessary to explore its detailed mechanisms and identify novel targets for the treatment of osteoporosis. Previously, we found that an lncRNA named GAS5 in humans could negatively regulate lipoblast/adipocyte differentiation. However, it is still unclear whether GAS5 affects osteoblast differentiation and whether GAS5 is associated with osteoporosis. Our current research found that GAS5 was decreased in the bones and BMSCs, a major origin of osteoblast, in osteoporosis patients. Mechanistically, GAS5 promotes osteoblast differentiation by interacting with UPF1 to degrade Smad7 mRNA. Moreover, a decreased bone mass and impaired bone repair ability were observed in Gas5 heterozygous mice, manifesting in osteoporosis. The systemic supplement of Gas5-overexpressing adenoviruses significantly ameliorated bone loss in an osteoporosis mouse model. In conclusion, GAS5 promot..., This dataset was obtained through various experiments, and statistical graphs were obtained after statistical analysis by SPSS software., , # GAS5 protects against osteoporosis by targeting UPF1/SMAD7 axis in osteoblast differentiation

[https://doi.org/10.5061/dryad.9cnp5hqfj](https://doi.org/10.5061/dryad.9cnp5hqfj)

By studying the GAS5 gene in MSCs cells and animals, we found that GAS5 can promote MSCs osteoblast differentiation and protect against osteoporosis by targeting UPF1/SMAD7. This dataset includes the study data and original images of the research.

## Description of the data and file structure

This dataset is divided according to each Figure. The raw data for each Figure corresponds separately to the folder in it. Images of the supplementary data included in the study are also presented in this dataset.

#### Staining Data

* ALP: alkaline phosphatase staining
* ARS: alizarin red staining
* HE: hematoxylin-eosin staining
* MASSON: Masson staining
* COL1: COL1A1 immunohistochemical staining
* OCN: BGLAP or Bglap immunohistochemical staining

#### Statistic Data

The statistical data of each experi...