Effect of ASC-J9 on gene expression of murine bone marrow-derived macrophages

The Androgen receptor (AR) in prostate cancer cells acts as a central transcription factor, playing a crucial role in maintaining tumour cell survival and the production of prostate-specific antigen (PSA).To investigate the effect of AR on macrophage gene phenotype, mouse bone marrow-derived macrophages (BMDMs) treated with the AR-degrading agent ASC-J9 in presence of conditioned media (CM) derived from RM1 mouse prostate cancer cells (RM1 CM) was collected for RNA sequencing (RNA-seq). Differential expressed genes (DEGs) analysis showed that compared to DMSO-treated macrophages, ASC-J9-treated macrophages had lower expressions of Trem2 and Trem1, anti-inflammatory M2-like macrophage markers Cd163 and Arg1 expression, pro-migratory factors Ccl2 and Ccl8, but had higher expressions of pro-inflammatory M1-like macrophage markers Cd86 and Tnf, suggesting that AR may be associated with macrophage phenotypic differentiation. RNA-seg profiling of C57BL/6J wildtype or TREM2 knockout (TREM2 KO) mouse bone marrow-derived macrophages treated with 10 μM ASC-J9 or DMSO for 48 h.