PTEN interacts with the transcription machinery on chromatin and regulates RNA polymerase II-mediated transcription.[RNA-seq and ChIP-seq]

We demonstrate that PTEN interacts with the transcription machinery. Using ChIP-seq, we show that PTEN co-localizes with RNAPII and binds to chromatin in promoter and putative enhancer regions. We further show that loss of PTEN affects RNAPII occupancy in gene bodies and correlates with gene expression changes. Loss of PTEN also increased cells’ sensitivity to transcription inhibition via small molecules. We interogate HeLa, MEF and MycOE mouse breast tumor cell lines for transcriptional changes after PTEN loss and correlate those with changes in PTEN, RNAPII, RNAPII Ser2P, RNAPII Ser5P occupancy at promoters, regulatory regions and in gene bodies.