ECOHAB: Kamykowski_D- Florida Process Cruise 11/16-19/1998- Karenia brevis counts, biochemistry and behavior (NODC Accession 0000534)

Abstract:
Water bottle samples were collected from selected depths
(variable with station) at 14 stations in the study area.
Coulter counts in the 14-28 um size class were routinely
determined for comparison with the microscope counts. Water
samples were filtered through 40 um Nytex mesh and
centrifuged for 10 minutes at 1200 x g to isolate Karenia
brevis. Pellets were frozen for subsequent analysis (pg
cell-1) of total lipid, neutral lipid, free amino acids,
protein and RNA. The biochemical determinations were
performed within two weeks of collection at Kamykowski's
NCSU laboratory. Aliquots were passed through 0.4 um
filters; the filters were placed in 90% acetone for
subsequent analysis of chlorophyll and the supernatant was
analyzed for nitrate.
Purpose:
To verify thet biochemical analyses of dense natural
populations of K. brevis can provide interpretable data on
cellular biochemical composition. To determine how the field
patterns relate to previously determined laboratory patterns
of depth-dependent biochemical composition.