Cellular and transcriptional characteristics of adipose derived stem cells from the ovine embryonic tail tissue reveals new regulators involved in adipogenesis

Adipose derived stem cells (ADSCs) enriched in adipose tissue with multilineage differentiation potential have become an important tool in therapeutic research and tissue engineering. Certain breeds of sheep exhibit a unique fat tail trait, where tail tissue accounts for approximately 10% of body weight and can provide an ideal source of ADSCs. In this study, using embryonic fat tail tissue of 80-day ovine embryo and collagenase I digestion, we isolated ADSCs and investigated their corresponding cellular and transcriptional characteristics. our results demonstrated that ovine ADSCs exhibited multilineage differentiation potential accompanied with morphological changes, including adipogenesis (for 6d), myogenesis (for 12d) and osteogenesis (for 24d). Furthermore, due to excellent adipogenesis ability of isolated ADSCs, we conducted bulk RNA-seq for differentiating ADSCs toward adipocyte at d0, d2, d4 and d6 to investigate its transcriptional profiling as well as molecular characteristics.