Iron-responsive genes in Lachancea kluyveri

To understand the iron-responsive gene expression in Lachancea kluyveri under high and low iron conditions, we perfomred the genome-wide gene expression profiling for the log-phase cells of Lachancea kluyveri wild type, sef1 deletion, aft1 deletion and sef1aft1 double deletion mutants under the YPD+400mM ferrous iron and YPD+ 200 mM BPS conditions. Cells fom overnight YPD cultures were diluted into YPD+400mM and YPD+200mM BPS at a cell density of 0.5 OD per ml and incubated at 30°C fro 5.5 h. Four biological replicates fro each strain under each condition were preprared with an exception of sample SF, which has only three biilogical replicates.