Single-cell RNA sequencing for vascular cell-enriched cell populations in the liver-specific apoE inducible mouse models

Vascular cell-enriched cells in the cortex of mice expressing apoE3 or apoE4 in the liver were isolated and subjected to the single-cell RNA seq to investigate the effects of peripheral apoE on the brain transcriptomic profiles. Our data revealed that liver-expressed apoE4 reduced astrocyte endfeet and promoted immune responses in the endothelial cells as welll as increases vessel-associated gliosis. Genes involved in IGF signaling, tight junction formation, actin cytoskeleton signaling, and maintenance of endothelial barrier function were up-regulated in the endothelial cells of mice expressing apoE3 in the liver. Four mice (2 males and 2 females) were analyzed for each of the four groups, including APOE3/Alb-Cre-, APOE3/Alb-Cre+, APOE4/Alb-Cre-, APOE4/Alb-Cre+.