Gene expression change in Keap1 knockdown (KD) mice in kidneys of ischemia-reperfusion injury (IRI)

We analyzed differences in IRI kidneys between WT and Keap1 KD mice (= Nrf2-activated mice). To identify Nrf2-target genes or metabolic genes in kidneys, we examined the mRNA expression profile both in normal (uninjured) and IRI kidneys (at day1 after unilateral IRI) from mice We performed microarray analyses using 1) Injured kidneys at day 1 after unilateral IRI, and 2) intact kidneys from mice which did not undergo UIRI. Samples were harvested from Keap1 KD mice and WT mice, n = 2 each,本研究分析了野生型（Wild Type, WT）小鼠与Keap1基因敲低（Knockdown, KD）小鼠（即核因子红细胞2相关因子2（Nuclear factor erythroid 2-related factor 2, Nrf2）激活型小鼠）的缺血再灌注损伤（Ischemia-Reperfusion Injury, IRI）肾脏之间的差异。为鉴定肾脏内的Nrf2靶基因或代谢相关基因，我们检测了小鼠正常（未损伤）肾脏及单侧缺血再灌注损伤后第1天的损伤肾脏的mRNA表达谱。本研究采用两类样本开展微阵列分析：1）单侧缺血再灌注损伤后第1天的损伤肾脏样本；2）未接受单侧缺血再灌注损伤的小鼠的完整肾脏样本。所有样本均取自Keap1基因敲低小鼠与野生型小鼠，每组样本量为2（n=2）。