Additional file 1 of Two lymphoid cell lines potently silence unintegrated HIV-1 DNAs

Additional file 1: Figure S1. Viral protein Vpr relieves silencing of unintegrated HIV-1 DNA. HeLa cells were infected with HIV-1 reporter virus expressing ZsGreen, either integration-competent (IN-wt, black curves) or integration-deficient (IN-D64A, red curves). A Vpr-positive reporter genome was generated by restoring the ORF sequence to the parent viral genome. Cells were scored by flow cytometry for % ZsGreen (upper panels) and Mean Fluorescence Intensity (MFI, lower panels). A Vpr-minus vector. B Vpr-positive vector.