Gene expression in IL-17RA-deficient and wild-type mice following Giardia muris infection

To elucidate IL-17A-dependent immune mechanims involved in regulating host defense, we employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential of regulating protective immunity that failed to be upregulated in the absence of IL-17RA signaling. Whole small intestine tissue collected from IL-17RA-deficient and C57BL/6 (wild-type) mice was collected 2 weeks after Giardia muris infection and from uninfected controls. Expression levels of several genes that may have anti-parasitic potential (Defb1, Retnlb, Saa1, Saa2) and may be involved in parasite clearance were, on average, lower or unchanged in IL-17RA deficient compared to wild-type mice after infection. Two weeks after Giardia muris challenge, total RNA was extracted and analyzed from tissue of the small intestines of infected IL-17RA-deficient and wild-type mice, and compared to uninfected controls. Each sample contained equal amounts of total RNA from 6 female mice which were pooled and used in the experiment.为阐明白细胞介素17A（IL-17A）依赖性免疫机制在宿主防御调控中的作用，我们采用全基因组微阵列表达谱作为发现平台，以筛选在白细胞介素17受体A（IL-17RA）信号缺失时无法上调、却具备调控保护性免疫潜力的基因。我们从肠贾第虫（Giardia muris）感染2周后的IL-17RA缺陷型与C57BL/6（野生型）小鼠中采集完整小肠组织，并设置未感染对照组。相较于感染后的野生型小鼠，IL-17RA缺陷型小鼠体内若干具备抗寄生虫潜力（Defb1、Retnlb、Saa1、Saa2）且可能参与寄生虫清除的基因，其表达水平整体呈下调或无显著变化趋势。在肠贾第虫攻毒2周后，我们从感染组IL-17RA缺陷型与野生型小鼠的小肠组织中提取总RNA并进行分析，并与未感染对照组进行比对。每份样本均由6只雌性小鼠的总RNA等量混合后制备，用于本实验。